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KMID : 0369319940140010065
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Allergy
1994 Volume.14 No. 1 p.65 ~ p.74
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Cross-reactivity among four cereals : rice, barley, wheat flour and buckwheat flour
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이기영
이수영/정병주/김규언/김동수
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Abstract
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There has been no report of rice allergy in Korea, but it is possible that rice allergy may be present in Korea. In this study, for the purpose of identification of safe cereals as a basic food in children with food allergy, we partially
purified
steammed rice, barley, wheat flour and buckwheat flour performed 12% gel SDS-PAGE and evaluated the cross reactivity among these four cereals by ELISA inhibition test with polyclonal rabbit IgG to four cerals.
The rice antigen contains at least 11 proteins. Among them a component of molecular weight with 66.2Kd was most prominently demonstrated by SDS-PAGE. Barley, wheat flour and buckwheat flour contain at least 11, 15 and 20 proteins, respectively.
Using the ELISA inhibition test, rice ELISA was inhibitted by other cereals, such as barley, wheat and buckwheat, up to 75.7%, 33.3% and 18.4%, respectively and significant inhibitions were noted barely and wheat flour (66.2% and 59.1%).
In conclusion, our result suggest that there was variable degree of cross reactivity among four common cereals in Korea and further study will be needed to identify the allergic cross reactivity among cereals in atopic children, especially with
hypersensitivity to at least one of four cereals, by use of IgG and IgE antibodies.
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KEYWORD
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